Hamas has had a base in the Qatari capital since 2012, reportedly at the request of the Obama administration. In anonymous briefings to Reuters, US officials said the Qatari government had agreed to tell Hamas to close its political office 10 days ago.
The reports have been denied by Hamas officials.
The small but influential Gulf state is a key US ally in the region. It hosts a major American air base and has handled many delicate political negotiations, including with Iran, the Taliban, and Russia. Alongside the US and Egypt, the Qataris have also played a major role in rounds of so-far unsuccessful talks to broker a ceasefire in the year-long war between Israel and Hamas in Gaza.
But there is growing evidence of a shift in the relationship.
After the killing of Hamas leader Yahya Sinwar, Hamas held a two-hour mourning tent in Doha in a small hall, a stark contrast to the recent three-day mourning held for Hamas leader Ismail Haniyeh, which was conducted with official state oversight and security.
The latest round of talks in mid-October failed to produce a deal, with Hamas rejecting a short-term ceasefire proposal. The group has always called for a complete end to the war and the full withdrawal of Israeli troops from Gaza.
Israel has also been accused of rejecting deals. Days after being fired earlier this week, former Defence Minister Yoav Gallant accused Israeli Prime Minister Benjamin Netanyahu of rejecting a peace deal against the advice of his security chiefs.